Inbred corn line FR3383

ABSTRACT

An inbred corn line, designated FR3383, is disclosed. The invention relates to the seeds of inbred corn line FR3383, to the plants of inbred corn line FR3383 and to methods for producing a corn plant produced by crossing the inbred line FR3383 with itself or another corn line. The invention further relates to hybrid corn seeds and plants produced by crossing the inbred line FR3383 with another corn line.

BACKGROUND OF THE INVENTION

The present invention relates to a new and distinctive corn inbred line,designated FR3383. There are numerous steps in the development of anynovel, desirable plant germplasm. Plant breeding begins with theanalysis and definition of problems and weaknesses of the currentgermplasm, the establishment of program goals, and the definition ofspecific breeding objectives. The next step is selection of germplasmthat possess the traits to meet the program goals. The goal is tocombine in a single variety or hybrid an improved combination ofdesirable traits from the parental germplasm. These important traits mayinclude higher yield, resistance to diseases and insects, better stalksand roots, tolerance to drought and heat, and better agronomic quality.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, and recurrent selection.

The complexity of inheritance influences choice of the breeding method.Backcross breeding is used to transfer one or a few favorable genes fora highly heritable trait into a desirable cultivar. This approach hasbeen used extensively for breeding disease-resistant cultivars. Variousrecurrent selection techniques are used to improve quantitativelyinherited traits controlled by numerous genes. The use of recurrentselection in self-pollinating crops depends on the ease of pollination,the frequency of successful hybrids from each pollination, and thenumber of hybrid offspring from each successful cross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested and compared toappropriate standards in environments representative of the commercialtarget area(s) for three years at least. The best lines are candidatesfor new commercial cultivars; those still deficient in a few traits areused as parents to produce new populations for further selection.

These processes, which lead to the final step of marketing anddistribution, usually take from eight to 12 years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a minimum of changes in direction.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior plant is to observe its performancerelative to other experimental plants and to a widely grown standardcultivar. If a single observation is inconclusive, replicatedobservations provide a better estimate of its genetic worth.

The goal of plant breeding is to develop new, unique and superior corninbred lines and hybrids. The breeder initially selects and crosses twoor more parental lines, followed by repeated selfing and selection,producing many new genetic combinations. The breeder can theoreticallygenerate billions of different genetic combinations via crossing,selfing and mutations. The breeder has no direct control at the cellularlevel. Therefore, two breeders will never develop the same line, or evenvery similar lines, having the same corn traits.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions, and further selections arethen made, during and at the end of the growing season. The inbred lineswhich are developed are unpredictable. This unpredictability is becausethe breeder's selection occurs in unique environments, with no controlat the DNA level (using conventional breeding procedures), and withmillions of different possible genetic combinations being generated. Abreeder of ordinary skill in the art cannot predict the final resultinglines he develops, except possibly in a very gross and general fashion.The same breeder cannot produce the same line twice by using the exactsame original parents and the same selection techniques. Thisunpredictability results in the expenditure of large research monies todevelop a superior new corn inbred line.

The development of commercial corn hybrids requires the development ofhomozygous inbred lines, the crossing of these lines, and the evaluationof the crosses. Pedigree breeding and recurrent selection breedingmethods are used to develop inbred lines from breeding populations.Breeding programs combine desirable traits from two or more inbred linesor various broad-based sources into breeding pools from which inbredlines are developed by selfing and selection of desired phenotypes. Thenew inbreds are crossed with other inbred lines and the hybrids fromthese crosses are evaluated to determine which have commercialpotential.

Pedigree breeding is used commonly for the improvement ofself-pollinating crops or inbred lines of cross-pollinating crops. Twoparents which possess favorable, complementary traits are crossed toproduce an F₁. An F₂ population is produced by selfing one or severalF₁'s or by intercrossing two F₁'s (sib mating). Selection of the bestindividuals is usually begun in the F₂ population; then, beginning inthe F₃, the best individuals in the best families are selected.Replicated testing of families, or hybrid combinations involvingindividuals of these families, often follows in the F₄ generation toimprove the effectiveness of selection for traits with low heritability.At an advanced stage of inbreeding (i.e., F₆ and F₇), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new cultivars.

Mass and recurrent selections can be used to improve populations ofeither self- or cross-pollinating crops. A genetically variablepopulation of heterozygous individuals is either identified or createdby intercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued.

Backcross breeding has been used to transfer genes for a simplyinherited, highly heritable trait into a desirable homozygous cultivaror inbred line which is the recurrent parent. The source of the trait tobe transferred is called the donor parent. The resulting plant isexpected to have the attributes of the recurrent parent (e.g., cultivar)and the desirable trait transferred from the donor parent. After theinitial cross, individuals possessing the phenotype of the donor parentare selected and repeatedly crossed (backcrossed) to the recurrentparent. The resulting plant is expected to have the attributes of therecurrent parent (e.g., cultivar) and the desirable trait transferredfrom the donor parent.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., Allard, 1960; Simmonds, 1979; Sneep et al., 1979; Fehr,1987).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, the grower, processor and consumer; for specialadvertising and marketing, altered seed and commercial productionpractices, and new product utilization. The testing preceding release ofa new cultivar should take into consideration research and developmentcosts as well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Once the inbreds that give the best hybrid performance have beenidentified, the hybrid seed can be reproduced indefinitely as long asthe homogeneity of the inbred parent is maintained. A single-crosshybrid is produced when two inbred lines are crossed to produce the F₁progeny. A double-cross hybrid is produced from four inbred linescrossed in pairs (A×B and C×D) and then the two F₁ hybrids are crossedagain (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁ hybrids islost in the next generation (F₂). Consequently, seed from hybridvarieties is not used for planting stock.

Corn is an important and valuable field crop. Thus, a continuing goal ofplant breeders is to develop stable, high yielding corn hybrids that areagronomically sound. The reasons for this goal are obviously to maximizethe amount of grain produced on the land used and to supply food forboth animals and humans. To accomplish this goal, the corn breeder mustselect and develop corn plants that have the traits that result insuperior parental lines for producing hybrids.

SUMMARY OF THE INVENTION

According to the invention, there is provided a novel inbred corn line,designated FR3383. This invention thus relates to the seeds of inbredcorn line FR3383, to the plants of inbred corn line FR3383 and tomethods for producing a corn plant produced by crossing the inbred lineFR3383 with itself or another corn line. This invention further relatesto hybrid corn seeds and plants produced by crossing the inbred lineFR3383 with another corn line.

The inbred corn plant of the invention may further comprise, or have, acytoplasmic factor that is capable of conferring male sterility. Partsof the corn plant of the present invention are also provided, such ase.g., pollen obtained from an inbred plant and an ovule of the inbredplant.

In one aspect, the present invention provides for single gene convertedplants of FR3383. The single transferred gene may preferably be adominant or recessive allele. Preferably, the single transferred genewill confer such traits as male sterility, herbicide resistance, insectresistance, resistance for bacterial, fungal, or viral disease, malefertility, enhanced nutritional quality, and industrial usage. Thesingle gene may be a naturally occurring maize gene or a transgeneintroduced through genetic engineering techniques.

In another aspect, the present invention provides regenerable cells foruse in tissue culture or inbred corn plant FR3383. The tissue culturewill preferably be capable of regenerating plants having thephysiological and morphological characteristics of the foregoing inbredcorn plant, and of regenerating plants having substantially the samegenotype as the foregoing inbred corn plant. Preferably, the regenerablecells in such tissue cultures will be embryos, protoplasts, meristematiccells, callus, pollen, leaves, anthers, roots, root tips, silk, flowers,kernels, ears, cobs, husks or stalks. Still further, the presentinvention provides corn plants regenerated from the tissue cultures ofthe invention.

DEFINITIONS

In the description and tables which follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

Predicted RM. This trait for a hybrid, predicted relative maturity (RM),is based on the harvest moisture of the grain. The relative maturityrating is based on a known set of checks and utilizes conventionalmaturity systems such as the Minnesota Relative Maturity Rating System.

MN RM. This represents the Minnesota Relative Maturity Rating (MN RM)for the hybrid and is based on the harvest moisture of the grainrelative to a standard set of checks of previously determined MN RMrating. Regression analysis is used to compute this rating.

Yield (Bushels/Acre). The yield in bushels/acre is the actual yield ofthe grain at harvest adjusted to 15.5% moisture.

Moisture. The moisture is the actual percentage moisture of the grain atharvest.

GDU Silk. The GDU silk (=heat unit silk) is the number of growing degreeunits (GDU) or heat units required for an inbred line or hybrid to reachsilk emergence from the time of planting. Growing degree units arecalculated by the Barger Method, where the heat units for a 24-hourperiod are: ${GDU} = {\frac{\left( {{Max}.{+ {Min}}} \right)}{2} - 50.}$

The highest maximum used is 86° F. and the lowest minimum used is 50° F.For each hybrid, it takes a certain number of GDUs to reach variousstages of plant development. GDUs are a way of measuring plant maturity.

Stalk Lodging. This is the percentage of plants that stalk lodge, i.e.,stalk breakage, as measured by either natural lodging or pushing thestalks determining the percentage of plants that break off below theear. This is a relative rating of a hybrid to other hybrids forstandability.

Root Lodging. The root lodging is the percentage of plants that rootlodge; i.e., those that lean from the vertical axis at an approximate30° angle or greater would be counted as root lodged.

Plant Height. This is a measure of the height of the hybrid from theground to the tip of the tassel, and is measured in inches.

Ear Height. The ear height is a measure from the ground to the ear nodeattachment, and is measured in inches.

Dropped Ears. This is a measure of the number of dropped ears per plot,and represents the percentage of plants that dropped an ear prior toharvest.

Allele. The allele is any of one or more alternative forms of a gene,all of which alleles relate to one trait or characteristic. In a diploidcell or organism, the two alleles of a given gene occupy correspondingloci on a pair of homologous chromosomes.

Backcrossing. Backcrossing is a process in which a breeder repeatedlycrosses hybrid progeny back to one of the parents, for example, a firstgeneration hybrid F₁ with one of the parental genotypes of the F₁hybrid.

Essentially all the physiological and morphological characteristics. Aplant having essentially all the physiological and morphologicalcharacteristics means a plant having the physiological and morphologicalcharacteristics, except for the characteristics derived from theconverted gene.

Quantitative Trait Loci (QTL). Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Regeneration. Regeneration refers to the development of a plant fromtissue culture.

Single Gene Converted. Single gene converted or conversion plant refersto plants which are developed by a plant breeding technique calledbackcrossing wherein essentially all of the desired morphological andphysiological characteristics of an inbred are recovered in addition tothe single gene transferred into the inbred via the backcrossingtechnique or via genetic engineering.

DETAILED DESCRIPTION OF THE INVENTION

Inbred corn line FR3383 is a yellow dent corn with superiorcharacteristics, and provides an excellent parental line in crosses forproducing first generation (F₁) hybrid corn.

FR3383 was developed from the cross of (44IEOV×39EOJK)×27JLPP utilizingpedigree breeding through ear to row advancement of selected selfedplants. Rigorous selection was placed on advanced progeny for favorableplant health and agronomic characteristics. Hybrid evaluation ofselected lines crossed to elite pollinators were conducted over multipleyears and environments within years. Yield, maturity, stalk quality,root quality, disease tolerance, late plant greenness, late plantintactness, ear retention, pollen shedding ability, silking ability andinsect tolerance were the criteria used to determine the rows from whichears were selected.

Inbred corn line FR3383 has the following morphologic and othercharacteristics (based primarily on data collected at Champaign, Ill.and/or Arcanum, Ohio).

VARIETY DESCRIPTION INFORMATION

1. Type: Dent

2. Maturity:

Days Heat Units From emergence to 50% of plants in silk: 76 1386 Fromemergence to 50% of plants in pollen: 77 1413 First Pollen DAP: 74 LastPollen DAP: 80 Pollen Duration  6

3. Plant:

Plant Height (ground to where top leaf is attached to plant): 53 inches

Ear Height (to base of top ear): 22 inches

4. Leaf:

Leaf Erectness: Semi-erect

5. Tassel:

Anther Color: Green

Glume Color: Yellow

6. Ear:

Ear Length: 6 inches

Number of Kernel Rows: 16

Endosperm Color: Yellow

7. Cob:

Cob Color: White

8. Disease Resistance:

Gray Leaf Spot (Cercospora zeae-maydis): Susceptible

Northern Leaf Blight (Exserohilum turcicum) Race 2: Susceptible

Southern Leaf Blight (Bipolaris maydis): Susceptible

Stewart's Wilt (Erwinia stewartii): Intermediate

Goss's Wilt (Clavibacter michiganense spp. Nebraskense): Intermediate

Northern Corn Leaf Spot (Bipolaris zeicola) Race 2: Susceptible

Northern Corn Leaf Spot (Bipolaris zeicola) Race 3: Susceptible

This invention is also directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plant,wherein the first or second corn plant is the inbred corn plant from theline FR3383. Further, both first and second parent corn plants may befrom the inbred line FR3383. Therefore, any methods using the inbredcorn line FR3383 are part of this invention: selfing, backcrosses,hybrid breeding, and crosses to populations. Any plants produced usinginbred corn line FR3383 as a parent are within the scope of thisinvention. Advantageously, the inbred corn line is used in crosses withother corn varieties to produce first generation (F₁) corn hybrid seedand plants with superior characteristics.

As used herein, the term “plant” includes plant cells, plantprotoplasts, plant cell of tissue culture from which corn plants can beregenerated, plant calli, plant clumps, and plant cells that are intactin plants or parts of plants, such as pollen, flowers, kernels, ears,cobs, leaves, husks, stalks, and the like.

The present invention contemplates a corn plant regenerated from atissue culture of an inbred (e.g., FR3383) or hybrid plant of thepresent invention. As is well known in the art, tissue culture of corncan be used for the in vitro regeneration of a corn plant. By way ofexample, a process of tissue culturing and regeneration of corn isdescribed in European Patent Application, publication 160,390, thedisclosure of which is incorporated by reference. Corn tissue cultureprocedures are also described in Green & Rhodes (I 982) and Duncan, etal., (1985). The study by Duncan et al., (1985) indicates that 97percent of cultured plants produced calli capable of regeneratingplants. Subsequent studies have shown that both inbreds and hybridsproduced 91 percent regenerable calli that produced plants.

Other studies indicate that non-traditional tissues are capable ofproducing somatic embryogenesis and plant regeneration. See, e.g.,Songstad et al., (1988); Rao et al., (1986); and Conger et al., (1987),the disclosures of which are incorporated herein by reference.Regenerable cultures may be initiated from immature embryos as describedin PCT publication WO 95/06128, the disclosure of which is incorporatedherein by reference.

Thus, another aspect of this invention is to provide for cells whichupon growth and differentiation produce the inbred line FR3383.

FR3383 is five days later shedding pollen and two days later silkingthan FR1064, a proprietary inbred corn line of Illinois FoundationSeeds, Inc. of similar usage as FR3383. Compared to the public inbredB73, FR3383 is one day later shedding pollen and one day earliersilking. Both FR1064 and FR3383 have a white cob color, but B73 has ared cob.

Some of the criteria used to select ears in various generations include:yield, stalk quality, root quality, disease tolerance, late plantgreenness, late season plant intactness, ear retention, pollen sheddingability, silking ability, and corn borer tolerance. During thedevelopment of the line, crosses were made to inbred testers for thepurpose of estimating the line's general and specific combining ability,and evaluations were run by the Champaign, Ill. and/or Arcanum, OhioResearch Station. The inbred was evaluated further as a line and innumerous crosses by other research stations across the Corn Belt. Theinbred has proven to have a very good combining ability in hybridcombinations.

The inbred has shown uniformity and stability. It has beenself-pollinated and ear-rowed a sufficient number of generations, withcareful attention to uniformity of plant type to ensure homozygosity andphenotypic stability. The line has been increased both by hand andsibbed in isolated fields with continued observations for uniformity. Novariant traits have been observed or are expected in FR3383.

Tables

In Table 1 that follows, the complete disease ratings for FR3383compared to other corn lines over several years. Disease ratings areshown for FR3383 in comparisons as an inbred to other inbred(s) and as ahybrid (first hybrid) to other hybrids. Data for Stewart's Wilt(Stewart) and Goss's Wilt (Goss) are shown as a 1 to 9 rating, with 1most resistant and 9 susceptible. Data for Northern corn Leaf Blight(NCLB), Gray Leaf Spot (GLS), Northern Corn Leaf Spot (NCLS) Races 2 and3, Southern Corn Leaf Blight (SCLB), and Multiple Leaf Blight (MLB) aregiven rating numbers which represent the percent of leaf area blighted(%LAB) evaluated and recorded at approximately 2 and 4 weeks afterflowering. The Multiple Leaf Blight is inoculated with a mixture ofraces and species of 10 different fungal leaf pathogens. All diseasereaction data points are the mean of two replications.

TABLE 1 Disease Rating NCLS NCLS SCLB Stewart Goss NCLB GLS Race 2 Race3 % LAB MLB 1998 Hybrid FR3383 × LH185 4.0 2.5 42.5 48.8 46.3 42.5 47.541.3 Pioneer 3489 2.8 2.8 47.5 53.8 45.0 46.3 52.5 54.4 Pioneer 34R063.0 3.0 47.5 55.0 48.8 46.3 55.0 55.6 1999 Hybrid FR3383 × LH185 1.5 2.531.3 38.1 23.8 38.1 Pioneer 3489 3.0 5.0 36.3 40.0 30.0 39.4 Pioneer33R87 4.0 5.0 41.9 40.0 26.3 39.4 1999 Inbred FR3383 2.5 2.5 35.6 48.841.9 46.3 FRB73 6.0 7.5 41.3 45.6 36.9 54.4 FR1064 5.0 2.0 35.6 48.136.3 53.8

In Tables 2 and 3 that follow, the traits and characteristics of inbredcorn line FR3383 are given in hybrid combination. The data collected oninbred corn line FR3383 is presented for the key characteristics andtraits. The tables present yield test information about FR3383. FR3383was tested in several hybrid combinations at numerous locations, withtwo or three replications per location. Information about these hybrids,as compared to several check hybrids, is presented.

The first pedigree listed in the comparison group is the hybridcontaining FR3383. Information for the pedigree in the tables belowincludes:

1. Yield (YLD) in bushels/acre is the actual yield of the grain atharvest adjusted to 15.5% moisture.

2. A mean for the percentage moisture (% M) for the hybrid across alllocations at harvest.

3. A mean of the yield divided by the percentage moisture (Y/M) for thehybrid across all locations.

4. The root lodging (RL) is the percentage of plants that root lodge;i.e., those that lean from the vertical axis at an approximate 30 degreeangle or greater would be counted as root lodged.

5. Stalk lodging (SL) is the percentage of the plants that stalk lodge,i.e., stalk breakage, as measured by either natural lodging or pushingthe stalks determining the percentage of plants that break off below theear. This is a relative rating of a hybrid to other hybrids forstandability.

6. Ear Height (EH) is measured from the soil level to the node at whichthe upper ear was attached and is expressed in inches.

7. Staygreen (SG) is a subjective rating on a scale of 1 to 7 during thefall. A rating of 1 represents a plant with no dead leaf tissue and a 7represents a plant with all leaf tissue dead. A rating of 1 is the mostdesirable.

8. Field Score (FS) is a subjective rating of a scale of 1 to 9 madejust prior to harvest. A rating of 1 represents a hybrid with a verygood plant integrity after senescence and a 9 represents a hybrid withvery poor plant integrity after senescence. A rating of 1 is the mostdesirable.

The series of hybrids listed under the hybrid containing FR3383 areconsidered check hybrids. The check hybrids are compared to hybridscontaining the inbred FR3383.

TABLE 2 1998 COMPARISONS at 15 Locations Pedigree YLD % M Y/M RL SL EHSG FS FR3383 × LH185 197 18.60 10.60 2 1 39 4.2 4.8 Pioneer 3489 19418.00 10.80 1 1 36 4.6 3.7 Pioneer 34R06 193 18.50 10.40 2 1 37 4.9 3.6

TABLE 3 1999 COMPARISONS at 49 Locations Pedigree YLD % M Y/M RL SL EHSG FS FR3383 × LH185 183 19.20 9.50 3 2 42 4.6 4.5 Pioneer 3489 17818.50 9.60 2 3 40 4.6 3.7 Pioneer 33R87 177 19.30 9.20 4 2 44 4.5 4.6

FR3383 has intermediate resistance or tolerance to Stewart's Wilt(Erwinia stewartii) and Goss's Wilt (Clavibacter michiganense spp.Nebraskense) (Table 1.).

In both 1998 (Table 2) and 1999 (Table 3) FR3383 crossed to the inbredLH185 (111 days to relative maturity {DRM}) is tested versus Pioneerhybrid 3489 (108 DRM). LH185 is a proprietary inbred corn line ofHolden's Foundation Seeds. In both years FR3383 hybrid was higheryielding with more moisture at harvest and a slightly lower Y/M. In bothyears the FR3383 hybrid had slightly more root lodging and similar stalklodging. FR3383 hybrid had a higher ear height than Pioneer 3489. FR3383hybrid had a slightly more favorable staygreen and a less favorablefield score than Pioneer 3489.

In 1998 (Table 2) and 1999 (Table 3) FR3383 crossed to the inbred LH185(111 DRM) was compared to Pioneer 34R06 (109 DRM) and Pioneer 33R87 (112DRM), respectively. Compared to Pioneer 34R06 the FR3383 hybrid washigher yielding, wetter, lower Y/M. FR3383 hybrid had similarstandability, better staygreen and less favorable field score thanPioneer 33R06. Compared to Pioneer 33R87 the FR3383 hybrid was higheryielding, with a greater Y/M. Both hybrids had similar standability,staygreen and field score.

When the term inbred corn plant is used in the context of the presentinvention, this also includes any single gene conversions of thatinbred. The term single gene converted plant as used herein refers tothose corn plants which are developed by a plant breeding techniquecalled backcrossing wherein essentially all of the desired morphologicaland physiological characteristics of an inbred are recovered in additionto the single gene transferred into the inbred via the backcrossingtechnique. Backcrossing methods can be used with the present inventionto improve or introduce a characteristic into the inbred. The termbackcrossing as used herein refers to the repeated crossing of a hybridprogeny back to one of the parental corn plants for that inbred. Theparental corn plant which contributes the gene for the desiredcharacteristic is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental corn plant to which the gene or genes from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol (Poehlman & Sleper,1994; Fehr, 1987). In a typical backcross protocol, the original inbredof interest (recurrent parent) is crossed to a second inbred(nonrecurrent parent) that carries the single gene of interest to betransferred. The resulting progeny from this cross are then crossedagain to the recurrent parent and the process is repeated until a cornplant is obtained wherein essentially all of the desired morphologicaland physiological characteristics of the recurrent parent are recoveredin the converted plant, in addition to the single transferred gene fromthe nonrecurrent parent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalinbred. To accomplish this, a single gene of the recurrent inbred ismodified or substituted with the desired gene from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphological,constitution of the original inbred. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross, one ofthe major purposes is to add some commercially desirable, agronomicallyimportant trait to the plant. The exact backcrossing protocol willdepend on the characteristic or trait being altered to determine anappropriate testing protocol. Although backcrossing methods aresimplified when the characteristic being transferred is a dominantallele, a recessive allele may also be transferred. In this instance itmay be necessary to introduce a test of the progeny to determine if thedesired characteristic has been successfully transferred.

Many single gene traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single gene traits may or may not betransgenic, examples of these traits include but are not limited to,male sterility, waxy starch, herbicide resistance, resistance forbacterial, fungal, or viral disease, insect resistance, male fertility,enhanced nutritional quality, industrial usage, yield stability andyield enhancement. These genes are generally inherited through thenucleus. Some known exceptions to this are the genes for male sterility,some of which are inherited cytoplasmically, but still act as singlegene traits. Several of these single gene traits are described in U.S.Pat. Nos. 5,777,196; 5,948,957 and 5,969,212; the disclosure of which isspecifically incorporated herein by reference.

A further aspect of the invention relates to tissue culture of cornplants designated FR3383. As used herein, the term “tissue culture”indicates a composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant. Exemplary types of tissue cultures are protoplasts, calli, plantclumps, and plant cells that can generate tissue culture that are intactin plants or parts of plants, such as embryos, pollen, flowers, kernels,ears, cobs, leaves, husks, stalks, roots, root tips, anthers, silk andthe like. In a preferred embodiment, tissue culture is embryos,protoplast, meristematic cells, pollen, leaves or anthers. Means forpreparing and maintaining plant tissue culture are well known in theart. By way of example, a tissue culture comprising organs such astassels or anthers, has been used to produce regenerated plants. (SeeU.S. Pat. Nos. 5,445,961; 5,322,789; 5,948,957 and 5,969,212, thedisclosures of which are incorporated herein by reference).

DEPOSIT INFORMATION

A deposit of the Illinois Foundation Seeds, Inc. proprietary inbred cornline FR3383 disclosed above and recited in the appended claims has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBoulevard, Manassas, Va. 20110. The date of deposit was Apr. 3, 2002.The deposit of 2,500 seeds were taken from the same deposit maintainedby Illinois Foundation Seeds, Inc. since prior to the filing date ofthis application. All restrictions upon the deposit have been removed,and the deposit is intended to meet all of the requirements of 37 C.F.R.§1.801-1.809. The ATCC accession number is PTA-4197. The deposit will bemaintained in the depository for a period of 30 years, or 5 years afterthe last request, or for the effective life of the patent, whichever islonger, and will be replaced as necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

What is claimed is:
 1. An inbred corn seed designated FR3383, a sampleof said seed having been deposited under ATCC Accession No. PTA-4197. 2.A corn plant, or parts thereof, produced by growing the seed of claim 1.3. Pollen of the plant of claim
 2. 4. An ovule of the plant of claim 2.5. The corn plant of claim 2, wherein said plant is male sterile.
 6. Atissue culture of regenerable cells of a corn plant of inbred lineFR3383, wherein the tissue regenerates plants capable of expressing allthe morphological and physiological characteristics of the inbred lineFR3383; a sample of said seed having been deposited under ATCC AccessionNo. PTA-4197.
 7. A tissue culture according to claim 6, the cells orprotoplasts being from a tissue selected from the group consisting ofleaves, pollen, embryos, roots, root tips, anthers, silks, flowers,kernels, ears, cobs, husks, and stalks.
 8. A method for producing ahybrid corn seed comprising crossing a first inbred parent corn plantwith a second inbred parent corn plant and harvesting the resultanthybrid corn seed, wherein said first or second parent corn plant is thecorn plant of claim
 2. 9. A hybrid corn seed produced by the method ofclaim
 8. 10. A hybrid corn plant, or parts thereof, produced by growingsaid hybrid corn seed of claim
 9. 11. Corn seed produced by growing saidhybrid corn plant of claim
 10. 12. A corn plant, or parts thereof,produced from seed of claim
 11. 13. A method for producing a hybrid cornseed comprising crossing an inbred plant according to claim 2 withanother, different corn plant.